O.S. Sychov, T.V. Mikhalieva, T.V. Talaieva, I.M. Gorbas, K.O. Mikhaliev, A.S. Zhukovska.Allelic polymorphism of connexin-40 gene (rs10465885) in patients with non-valvular atrial fibrillation

The aim – to study the allelic polymorphism of connexin-40 gene (rs10465885) in patients with non-valvular atrial fibrillation (AF) in the Ukrainian population.

Material and methods. We enrolled 112 patients (mean age (50±10) years; males – 86 (76,8 %), females – 26 (23,2 %)) with non-valvular AF, associated mainly with essential hypertension (EH), coronary heart disease (CAD), EH and CAD constellation, and myocardial fibrosis. Among them 47 (42,0 %) patients had paroxysmal AF, 45 (40,2 %) – persistent AF and 20 (17,8 %) – permanent AF. We also enrolled 78 age- and gender-matched healthy controls (60 males (77 %), 18 females (23 %); mean age (51±11) years) with CAD risk factors. The single nucleotide polymorphism rs10465885 (Т–26→С) in promoter B connexin-40 gene was genotyped by means of real time polymerase chain reaction (T – reference («wild» type) allele, C – minor allele).

Results. Genotype and allelic distribution of rs10465885 in patients with AF was as follows: T/T – 25,9 %; C/T – 49,1 %; C/C – 25,0 %; allele T – 50,5 %; C – 49,5 %. The distribution in controls was as follows: Т/Т – 28,2 %; С/T – 41,0 %; C/C – 30,8 %; allele Т – 48,7 %, С – 51,3 %. Distribution of rs10465885 was comparable in both AF patients and controls. Genotype C/T frequency in AF females was higher than in control females (69 % and 28 %, respectively).

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