V.M. Kovalenko, T.V. Talaeva, A.S. Kozlyuk.Effect of systemic enzyme therapy treatment on components of the insulin resistance syndrome in experimental model

The aim – to evaluate the dependence between inflammatory intensity and metabolic disorders and efficacy of systemic enzyme therapy (SET) in their correction in experimental condition.
Material and methods. We examined 40 rabbits which were kept on lipid-enriched diet for 8 weeks: 10 rabbits which took systemic enzyme therapy from 1st day of lipid-enriched diet (1st group), 10 rabbits which took systemic enzyme therapy from 5th day of experiment (2nd group) and 20 rabbits without treatment. At the beginning of the study and after 4th and 8th week blood of rabbits was examined on inflammatory intensity (with measurement of C-reactive protein (CRP), intracellular malondialdehyde (MDA), monocytes activity (MC)), oxidative stress intensity (MDA in serum), renin-angiotensin system activity with measurement of angiotensin converting enzyme activity (ACE act.). In addition, we studied lipid metabolism by measuring cholesterol and triglycerides blood levels, main classes of lipoproteins and their atherogenic potential (concentrations of cholesterol in circulating immune complex (CIC) and triglycerides in CIC), levels of glucose and HbAlc. Besides, tissue sensivity to insulin was evaluated in all rabbits.>br />Results. It was found that at the group without treatment CRP level was 18 times elevated, MDA MC level and MDA ser. level became 5.0 and 7.4 times higher respectively at the end of the 8th week in comparison with baseline (Р<0.001). ACE activity increased by 150 % (Р<0.001). Tissue sensitivity to insulin decreased nearly by 86 %, and as a result, glucose concentration in blood increased by 90 %, HbAlc level increased 3.7 times at the end of the study (Р<0.001). As a result of lipid peroxydation of triglycerides and cholesterol and elevation of their atherogenic potential, concentration in CIC were 5.6 and 4.5 times higher in comparison with control group (Р<0.001). At the 1st group we found 4 times lower levels of CRP (Р<0.001), 57 % lower concentration of MDA MC and in 3.7 times lower concentration of MDA ser. Levels of TG CIC and cholesterol in CIC decreased by 64 and 62 %, respectively, in comparison with group without treatment (Р<0.001).
Conclusion. Keeping rabbits on lipid-enriched diet resulted in development of disorders typical for metabolic syndrome. Using systemic enzyme therapy from 1st day of high-lipid diet lead to reduction of systemic inflammation intensity and immune reactivity, restoration of tissue sensitivity to insulin, normalization of blood lipid composition and reduction of its atherogenic characteristics. Therapeutic effects of SET increased during period of experiment.

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