Оne of the most powerful tools for health-related quality of life (HRQOL) assessment in patients with ischemic heart disease (IHD) is MacNew Heart Disease Health-Related Quality of Life Questionnaire (MacNew). Correct translation to other languages plays key role in getting adequate HRQOL assessment tool. To confirm language-specific equivalence it should be validated before use in clinical practice or research. MacNew Heart Disease Health-Related Quality of Life Questionnaire validation in Ukrainian-speaking patients was performed in the Volyn Regional Center of Cardiovascular Diseases as a part of the HeartQoL Project. Totally 322 patients with IHD (68.9 % men, mean age 58.1±8.9 years) were included into the study. Among them, 105 survived myocardial infarction (MI), 111 had angina pectoris and 106 – heart failure (HF). All patients completed the MacNew, SF-36, HADS questionnaires. In addition, 15.0 % of patients (random sample) filled them again in two weeks. Questionnaires were translated into Ukrainian by double forward and backward translation for using in Ukrainian patients. According to the MacNew questionnaire, patients with HF had the lowest level of HRQOL in physical and emotional scales compared to patients with angina and after MI. No significant difference between groups was found in SF-36. The mean levels of anxiety (7.0±3.6) and depression (6.1±4.1) were similar in patients with HF, angina and MI. Dispersion analysis of the validity assessment of MacNew whole and its physical and emotional scales demonstrated significant direct relationship compared with generalized dynamic assessment of health by SF-36 and levels of anxiety/depression by HADS. Internal consistency reliability and test-retest reliability of whole MacNew, and the analysis in scales, and depending on the diagnosis, was ≥ 0.90. This confirmed validity hypotheses. In conclusion, the Ukrainian version of the MacNew is reliable and valid in patients with angina, HF and in MI survivors. It may be recommended for use in assessing the Health Related Quality of Life in Ukrainian patients with IHD.