The aim of the study was to examine the role of speckle tracking echocardiography in assessment of systolic and diastolic function, to establish reference values of global and segmental longitudinal, radial, and circumferential strain as well as longitudinal, radial, circumferential strain rate and to study the myocardial deformation according to sex in a healthy population. A total of 33 healthy individuals (10 men, 23 women), mean age (26.7±0.5) years were observed. Global longitudinal, radial, and circumferential strain as well as global longitudinal, radial, circumferential strain rate values were analyzed. Diastolic SR signals were recorded during early filling and at the end of diastole. The peak early filling (E-wave) and late diastolic filling (A-wave) velocities, the E/A ratio, left ventricular ejection fraction by Simpson’s biplane method, average value of the peak systolic mitral annular velocity by tissue Doppler imaging and end-systolic volume index have been estimated as well. The reference values for global and segmental longitudinal, circumferential, radial strain and strain rate in healthy persons were determined. Longitudinal strain and strain rate play a great role in the systolic function of the left ventricle.